Parametric determination of conditions for bioproduction of citric acid

The optimization of parameters, viz. molasses concentration, pH, temperature and incubation period have been studied for parametric determination of citric acid by Aspergillus niger NCIM 953. It has been found that production of citric acid by Aspergillus oryzae-368 proceeds best when molasses solution 20.5% (w/v) is allowed to ferment for 12 days of incubation period at 300C temperature by maintaining the pH value of fermenting medium to 2.2 along with other nutritional ingradients required by the fungus Aspergillus oryzae-368.