Cytokinin - mediated enhancement of molasses fermentation for ethanol production by Saccharomyces cerevisiae - 3078

The present study investigates the influence of the synthetic cytokinin derivative 6-(4- hydroxy-3-methyl-trans-2-butenyl) aminopurine on ethanol bioproduction from molasses using Saccharomyces cerevisiae-3078. Molasses, a low-cost agro-industrial byproduct rich in fermentable sugars, was employed as the primary carbon source. Exposure of yeast cells to the cytokinin analog significantly modulated cellular metabolism, resulting in improved sugar utilization, enhanced growth kinetics, and higher ethanol yields compared to untreated controls. The cytokinin-mediated response was associated with stimulation of glycolytic flux and a reduction in byproduct accumulation, thereby improving fermentation efficiency. Optimization studies revealed that moderate concentrations of the cytokinin elicited the most pronounced effect, while higher levels exerted inhibitory responses. These findings demonstrate the potential application of cytokinins as novel regulatory agents for improving microbial fermentation performance. This approach highlights an eco-friendly and cost-effective strategy for augmenting bioethanol production from molasses, offering prospects for industrial-scale bioprocess optimization. In the present investigation effect of 6-(4-hydroxy-3- methyl-trans--2-butenyl) aminopurine on production of ethanol from molasses by Saccharomyces cerevisiae3078 has been assessed. It has been observed that the cytokinins under trial has stimulatory effect on production of ethanol and enhances the yield of ethanol to an extent of 7.391% higher in comparison to control when 20%molasses solution (w/v) is allowed to ferment at pH 5.7,temperature 300C and incubation period of 50 hours along with some other significant rich ingredients required by the yeast Saccharomyces cerevisiae-3078.