Efficacy of some fluorogenic AMC compounds on bioproduction of citric acid

Fluorogenic AMC compound, i.e., 7- amino-4-methylcoumarin can be used to monitor enzyme production during fermentations, allowing for optimization of fermentation condition. In fermentation technology, various compounds can serve as substrates, inhibitors or modifiers of enzyme activity. The efficacy of 7-amino-4-methylcoumarin (AMC) on bioproduction of citric acid by GRAS fungal strains such as Aspergillus awamori NCIM-1869, A. foetidus NCIM-1859, A. nidulans NCIM-1651, A. oryzae NCIM-1756 and Aspergillus niger NCIM-1856 has been studied. It has been found that A. niger NCIM-1856 is best producer of citric acid. The compound AMC has beneficial impact on production of citric acid by A.niger NCIM1856 and upgraded the yield of citric acid to an extent of 8.421% higher in comparison to control under the optimized parameters.