Exploring the role of 5,5'-diethyl hydantoin in enhancing efficiency of lactic acid bioproduction by S. lactis-2114

The growing demand for sustainable bioproduction of lactic acid has stimulated interest in exploring chemical modulators that can enhance microbial efficiency. In the present study, the role of 5,5'-diethyl hydantoin was investigated in improving lactic acid bioproduction by Streptococcus lactis 2114. Controlled fermentations were carried out with sucrose as the carbon source, both in the absence and presence of graded concentrations of 5,5'-diethyl hydantoin. The results demonstrated that the compound exerted a stimulatory influence on microbial growth kinetics, carbohydrate uptake, and lactic acid yield, with a notable reduction in by-product formation. Enhanced enzymatic activity of lactate dehydrogenase (LDH) and improved redox balance were observed, suggesting that 5,5'-diethyl hydantoin facilitated intracellular metabolic regulation. At the optimized concentration, lactic acid productivity increased significantly compared to control conditions, highlighting the compound’s potential as an efficiency enhancer in microbial fermentation processes. These findings provide new insights into the application of hydantoin derivatives as modulators of microbial metabolism, opening avenues for costeffective and scalable lactic acid bioproduction. The compound 5,5'-diethyl hydantoin was found stimulatory when 20% (w/v) molasses solution was allowed to ferment at pH level 6.0, temperature 43°C and optimum incubation period of 144 hrs. The maximum yield of lactic acid was found to be 6.42292 g/100mL at 4.0×10–4 M molar concentration of 5,5'- diethyl hydantoin which is 24.924% higher in comparison to control fermentor flasks. In the present communication the authoress has confined her study to explore the role of 5,5'-diethyl hydantoin in enhancing efficiency of lactic acid by S.lactis-2114.