Regulatory role of 5,7-dihydroxy -4-propylcoumarin in citric acid bioproduction by Aspergillus oryzae NCIM-929

The regulation of secondary metabolite pathways through small bioactive molecules offers promising strategies for enhancing organic acid bioproduction. In the present study, the regulatory role of 5,7-dihydroxy-4-propylcoumarin in citric acid production by Aspergillus oryzae NCIM-929 was investigated. Coumarin derivatives, known for their antioxidant and signaling activities, have been suggested to influence metabolic fluxes in filamentous fungi. Supplementation of 5,7-dihydroxy-4-propylcoumarin at optimized concentrations led to a significant increase in citric acid yield, accompanied by improved substrate assimilation efficiency and altered intracellular redox balance. These findings highlight the potential of 5,7-dihydroxy-4- propylcoumarin as a metabolic regulator for optimizing citric acid bioproduction in A. oryzae and provide a novel approach for biotechnological applications of natural small molecules in industrial fermentation. In the present communication regulatory role of 5,7-dihyroxy-4-propylcoumarin in citric acid bioproduction by Aspergillus oryzae NCIM-929 has been studied. It has been observed that 5,7-dihydroxy-4-propylcoumarin has stimulatory effect on bioproduction of citric acid and enhances the yield of citric acid to an extent of 15.399% higher in comparison to control when molasses solution of 24% was allowed to ferment at pH 2.0,temperature 270C and incubation period of 11 days.